ECON-E 201 Study Guide - Quiz Guide: His3, Tryptophan, Wild Type
67
ECON-E 201 Full Course Notes
Verified Note
67 documents
Document Summary
Lab day ___________________: why can complementation only be done with recessive mutations? (1 point, if wild-type allele cannot rescue the mutant phenotype, we will not be able to determine whether two mutants complement one another or not. We will not know how many genes are present: which of the following will you use to do your yeast matings today. Why or why not? (2 points: set-up f1 x f1 crosses with siblings from the 3a experiment. Since all sibs are the same genotype, using virgins is not necessary: you have isolated a group of 8 recessive, tryptophan- auxotrophs in yeast and wish to determine how many different genes have been mutated. Describe how you could accomplish this task. (3 points: generate a" and " mating type forms of each mutant, mate each strain to every other strain, plate the diploid strains on trp- minus media. If they fail to grown, then the mutations are in the same gene.
