BIOL-M 315 Study Guide - Midterm Guide: Pyocyanin, Thymol, Botulism

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Plug cord into electrical outlet; flip on-off switch to on (23) Set light intensity control (3) ~ turn brighter than the minimum. Using the nose piece (13) swing the lowest power objective (5x-red) until it clicks into place. Adjust the oculars (15) so you see one circle of light with both eyes. Raise the condenser (8 or 20) to the most upward position. Be sure the condenser turret (11) is set in the bf position on the front side of the condenser. Look through the oculars and adjust the condenser diaphragm (11-black lever on top) to give a comfortable amount of light (~ 1/2 open, lever in the center) Place the slide onto the stage (12); open the slide holder by rotating the clip to the left; insert the slide all the way back; release the clip. Center the specimen over the condenser lens using the two stage adjustment knobs (6 and 7)