BIOL 2300 Study Guide - Midterm Guide: Bioremediation, Acidophile, Counterstain
Document Summary
Observed the bacteria in lake water through a magnifying glass o. Four techniques that showed that specific microorganisms are linked to specific diseases. Considered to be father of modern microbiology. 1861 performed experiments disproving spontaneous generation swan-necked flask. Clean and sterile environment to decrease post surgery: fleming: infection. Ames test: a method that uses bacteria to determine whether a chemical can cause mutations in dna. Microscopy: total magnification: ocular x objective lens, refractive index: the ratio of the velocity to its velocity in a specified medium. Microscope: going from glass to liquid: resolution: quality of lens/ illuminating source. Shorter wavelength: better resolution: application of various microscopes: Bright-field: dark images against a brighter background. Dark-field: light is scattered by the sample. Absorb uv (short wavelength) and emit lower energy light (long wavelength) 3 beams of lights and prism to view image. Scanner laser: sends laser through sections of an organism. Details of the internal structure of the organism.
