BIOL 3110 Study Guide - Final Guide: Methylation, Uracil, Processivity
Document Summary
The synthesis of nucleotides are regulated by several feedback mechanisms. Dna polymerization occurs by a specific molecular mechanism. Methylation of dna occurs within bacterial cells some mutants lack this ability. Genomic and cdna placed in to phage or plasmid vectors (dependant on size) by ligation and then transformed into cells. Selection is determined by lac z and amp resistance. High salt, low temp = high or low. 2 ways linkers and adding a re to primers then ligate etc. If 2 different targets existed with an equal amount of dna, would you expect qpcr to elicit similar responses for each? (ie would you notice the pcr product at the same number of cycles?) Genome/dna structure can be affected by many things like base twisting, ant/syn glycosidic bonds, supercoiling, pallindromic sequences, etc. Lots of acronyms used in this course mar, ori, orc, mcm, cdk, yac, bac, We"ve studied many diseases and their molecular basis.
