Biology 2581B Study Guide - Final Guide: Cloning, Phenotypic Trait, Tree Breeding
23 Apr 2016
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Groodies are fictional: wild type = polygamous, mutant groodies = monogamous. Positional cloning: looking for a gene responsible for a specific phenotype. Known location of microsatelite markers and the dna sequence surrounding them. In order to look for a gene that is responsible for a phenotype you need a mutant which indicates a physical change. Pcr, restriction enzymes, and any technique you know. This says that the monogamous trait is recessive. When you cross several you get tree breeding = homozygous. They are all monogamous so this suggests that they are a result from spontaneous reaction. Mutation is in multiple genes that each affect the polygamous phenotype. Females m1 m2 m3 m4 m5 m6 m7. This shows that only one gene determines the phenotype. This shows that there are two genes responsible: complementation is seen, and that trait is recessive. With this example, there is no complementation between the two genes, so the offspring will all me monogamous.
