Biology 2581B Study Guide - Quiz Guide: Phase-Contrast Microscopy, Fluorescence Microscope, Confocal Microscopy

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The only way to visualize them is therefore with the help of microscopy. Multiple things are important in this process, like the preparation of the specimen and the performance of a specific microscope. The choice of these depends on what you are looking at and the image you are willing to get. Light microscopy: can see things up to about 0. 2 um (wavelength the smallest): bright field microscope: uses light that passes directly through the cell to form an image. Staining required (if the sample does not have a colour itself). Only the surface of the sample can be seen. Samples need to be thin: phase contrast microscope: uses phase differences of light while it passes through the cell and increases these differences to create an image of the cell"s structure. Can visualize mitosis and cell migration (movement of living cells).

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