Biology 2581B Study Guide - Midterm Guide: Haemophilia B, Haemophilia A, Molecular Cloning
6 Mar 2017
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Positional cloning: process of finding the chromosomal location of a gene based on its phenotype. The gene responsible for hemophilia a was found without mapping information if hemophilia a, they are also color-blind it is different for hemophilia b, color-blindness inherited differently (cid:862)old(cid:863) interest: famous historical example. Queen in england was a carrier (cid:862)new(cid:863) interest. Haemophilia patients received untested and unscreened clotting factor prior to 1992. Extreme risk for contracting hiv and hepatitis c. Identify the effects of the mutation from a compiled list of factors btw wt and mutant. Discovered the loss of factor 8: purify factor 8, protein sequencing. Very expensive and hard so just a small piece is identified. ** note that aa"s (cid:272)ould (cid:271)e (cid:272)oded (cid:271)(cid:455) (cid:373)ultiple (cid:272)odo(cid:374)s (wobble) therefore, some ambiguity: synthesize degenerate probes. Degenrate: a mixture of similar, but not identical sequences. Making all possible options: genomic library.
