Biology 2382B Study Guide - Midterm Guide: Green Fluorescent Protein, Phase-Contrast Microscopy, Scanning Electron Microscope
25 Oct 2016
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Cell culturing is a technique used to grow cells/tissues in strictly controlled conditions. Cells are held together with proteins and carbohydrates. Trypsin is an enzyme that will essentially shear all of the p(cid:396)otei(cid:374)s f(cid:396)o(cid:373) the outside of (cid:272)ells, a(cid:374)d (cid:272)auses the(cid:373) to (cid:271)e less (cid:858)sti(cid:272)k(cid:455)(cid:859). Edta is another (cid:272)o(cid:373)pou(cid:374)d used that (cid:449)ill (cid:858)sop up(cid:859) all of the (cid:373)etal io(cid:374)s i(cid:374) the e(cid:454)t(cid:396)a(cid:272)ellula(cid:396) (cid:373)at(cid:396)i(cid:454), (cid:449)hi(cid:272)h fu(cid:396)the(cid:396) aids i(cid:374) separating cells. Once we have individual cells we need to place them onto a media. The media contains amino acids, minerals, vitamins, salts, glucose, as well as serum (containing insulin/growth factors). Further, the media needs to be kept at proper temperature conditions. Oxygen and co2 concentrations must also be used. The tissue media is red because it also is sensitive to ph. Purple means the ph is too low, yellow is too high. They also have magnetic stirrers, keeping the cells from adhering to the bottom of the culture vessel.
