Biochemistry 2280A Study Guide - Quiz Guide: Haematoxylin, Photon, Plant Cell
11 May 2020
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Parts of the microscope atom (electron microscope) small molecule (electron microscope) bacterium (light microscope) plant cell (light microscope) frog egg (eye) Different cells in a tissue or different organelles in a cell will selectively absorb light with different wavelength. For living organisms, you need a light microscope with divergent light waves. Phase shifts due to density in specimen, no fixation needed. When a photon with the correct energy hits a fluorescent molecule, an electron is excited to an orbital with a higher energy state. In this exited state the electron moves rapidly to an orbital with a slightly lower energy state. Finally the electron falls back to the ground state and the residual energy is emitted as light. The emitted light has lower energy and thus a longer wavelength. Express the fluorescent protein in a gene. Copple gfp at the gene coding for protein x. How are cells connected in a tissue. When and where is a gene expressed.
