BIOL 2111 Study Guide - Final Guide: Ethidium Bromide, Null Hypothesis, Dna Replication
16 May 2013
School
Department
Course
Professor
Document Summary
2 hours long, short answer format, 50 marks, bring calculator. Not memorizing, mostly understanding the concepts and how to apply them. Important to understand the solutions (wizard kit, ethidium bromide, etc) In this step, denaturation occurs (this step is called denaturation). This step is called annealing. The primers attach to where they are complimentary on the 2 strands. There is a forward primer on one strand and a reverse primer on the other strand. The primers attach and set up where the dna polymerase is going to replicate the dna. Taq adds free nucleotides (called dntps) to the dna strand. Taq attaches dntps to their complimentary bases. This is how we amplify and get copies of dna. Taq pulls in the nucleotides and they attach complimentary to the previous starnd of dna. Pcr won"t work without primers! Ex: what is the probability of rolling a 1 and a 5 on a die (1/6 x 1/6 =
