BIOL 230- Midterm Exam Guide - Comprehensive Notes for the exam ( 44 pages long!)
11 Oct 2017
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Document Summary
Omix: genomics is the analysis of entire genomes. Transcriptome analysis is the study of all expressed genes. The first vectors are big vectors (bacs or yacs). Now you take the individual bac and chop it up again into smaller pieces. This assembles entire chromosome: contigs: this is how you can assemble the whole genome. Contig 1 has an overlap with contig 2 and you can piece them all back together. This is how the human genome started but this is a lot of work: shotgun sequencing: in 1995 the first completed genome sequence from a free-living organism. This takes a whole genome, do a digest (cut into diff parts), but now we do not clone anything. Do not put anything in any vectors anymore. It is more challenging, it is random, every piece can be from everywhere but it saves time because you do not have to do any cloning or maintenance of clones.
