BIOB11H3 Study Guide - Midterm Guide: Centrifugation, Transcription Factor, 28S Ribosomal Rna
Document Summary
Wild-type neurospora: can survive in minimal medium (minimal medium: contain some inorganic salts, sugars, vitamins, cs) Able to undergo complex pathways using these simple ingredients to form macromolecules w/ enzymes encoded by their genome. Supplemented medium: enriched media with more complex material. Shows that daughters of the radiated spores can only grow on enriched. Medium but not the minimal medium o(cid:373)e (cid:373)utatio(cid:374) (cid:373)ust(cid:859)(cid:448)e o(cid:272)(cid:272)urred. Test with minimal medium + only one of the thing within the supplemented. Medium shows that it has deficiency in vitamin biosynthesis (for vitamin pantothenic acid specifically in this case) If enz2 gene mutated no enz2 to convert precursor b to c. Providing cells with c will (cid:858)res(cid:272)ue(cid:859) the specific mutation, but providing a or b will not (no enzyme 2 to work through anyways) Later, beadle & tatu(cid:373)(cid:859)s results lead to (cid:862)o(cid:374)e ge(cid:374)e-o(cid:374)e e(cid:374)zy(cid:373)e(cid:863) hypothesis. Where dna is transcript into rna by rna polymerases and other enzymes.
