CHM110H5 Study Guide - Final Guide: Paper Chromatography, Metal, Reagent
Document Summary
Chromatography allows the components of a mixture to be separated by their polarity and solubility when placed in a solvent. The purpose was to identify an unknown substance"s metal cations by comparing it to other known substances by paper chromatography. There are variations in each metals solubility, their attraction to the chromatography paper, and density (toon, ernest), which all result in the samples moving at different rates. The substances would then be identified by comparing the locations of the samples and using a reagent that is likely to be the same substance, and if the colour matches, that substance would be identified. The solvent must be poured into a beaker below 1. 5cm. The beaker is then covered with a plastic wrap. Ensure that the ammonia chamber is set up with plastic wrap covering the large beaker and the smaller beaker of 5ml of ammonia solution.
