KIN217 Study Guide - Catalytic Triad, Endopeptidase, Elastase
Document Summary
One by definition a catalyst can be reused the rest of the information is irrelevant: mechanism of the chymotrysin reaction. It hydrolyses peptide bonds adjacent to bulky hydrophobic groups. peptidase this means it acts on a peptide sequence and endo means inside exo on the outside . The catalytic triad (diagram in text, but number unknown: we see the serine residue up at the top note that the residues are linked linearly . The aspartate residue of the catalytic triad (fig 6. 11) The negative charge (?) is holding the hydrogen down . The catalytic triad is not restricted to chymotrypsin . Can function as a proton donor and as an acceptor (we are talking about the hydrogen on the southern side of the molecule) Elastase has a hydrophobic pocket but is much more restricted so the phenol group can"t fit in there! Have cysteine at the active site rather than serine. One donates a proton, the other accepts one.
