BIOL240L Study Guide - Quiz Guide: Focus 3, Methyl Blue, Oil Immersion
2 Oct 2017
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Does allow differentiation of cell types: cocci, rods, spirals, vibrios. Positive: stain is basic, has a cationic chromophore (colored part of molecule). attracted to outside of bacterial cells which are negative. slide background is white, cells are colored. Note: good practice to re-suspend bacteria in tubes by tapping with finger. turbidity or cloudiness in broth indicates bacteria growth. Note: objective lenses are near the stage, the ocular lenses are in the eyepieces. 1000 / actual size of clusters/packets (staphylococcus). does not produce spores produce spores. Coccus: round, non-motile, single (micrococcus), double (diplococcus), chains (streptococcus), Bacillus: rod, most motile, single, pairs (diplobacillus), chains (streptobacillus). some species. Spirillium: spiral, mostly motile, single, spirochetes. no spores. Rhodospirillum rubrum - collection of tiny c-shaped hairs - spirillium. Used to differentiate types of bacteria (differential staining technique) primary stain (called a mordant) First step is to stain with primary stain crystal violet, followed by gram"s iodine which fixes the.
