BIOL130 Study Guide - Final Guide: Spermatogenesis, Semiconservative Replication, Hematocrit
19 Sep 2014
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4 types of macromolecules + tests used for identification. Background information for tests (how do they work?) Know the difference between macromolecule suspended in solution (it is dissolved) vs. a macromolecule in suspension (it is not dissolved) Be familiar with the chemicals used in each process during isolation (ex. Nacl was used because nucleic acids are soluble in it) Know the parts of a chromatogram (stationary phase? mobile phase?) Solvent used for protein chromatogram and solvent used for nucleic acid chromatogram. Do you understand how to use a concentration curve to find the concentration of an unknown substance? (likely exam question) Do you understand how to calculate concentration? (c1v1 = c2v2) ex. You have a stock red solution with a concentration of 0. 01 mg/ml. You add 5 ml of water and 5 ml of this stock solution into a test tube. Parts of the spectrophotometer and their role (white light source, prism, narrow slit, cuvette, photoelectric cell, galvanometer)
