MBB 222 Study Guide - Final Guide: Transcription Bubble, Pyrophosphate, Dna Replication
14 Nov 2016
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Nascent = freshly generated in a reactive form. The nascent polymer for dna replication is a polydeoxyribonucleotide and for transcription it is a polyribonucleotide. The dna strand that does not get transcribed. It is the coding strand because the mrna transcript is the same as this strand except that it has thymine instead of uracil. The region on an enzyme surface that binds the substrate molecule and catalytically transforms it. U1-07 must mechanism / pathway / reaction steps. A mechanism explains in detail how the process occurs at the smallest level with atoms and molecules, the following are 2 such examples: Catalytic mechanism of adding a new nucleotide by dna polymerase. Involves: 2 mg2+ ions (coordinated to the phosphate groups of the incoming ntp), the 3"-hydroxyl group (acts as the nucleophile), and 3. Mg2+ facilitates the attack of the 3"-hydroxyl group to the alpha phosphate on the pyrophosphate.
