BCH 361 Study Guide - Final Guide: Yeast Extract, Ethanol Fermentation, Fructose-Bisphosphate Aldolase
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What should he have done differently: what should stanley do in his experiment, you will be given additional questions in class relating to stanley"s experiment. Additional questions for study or discussion, time permitting. In the liver, fructose can be converted into gap and dhap without passing through the phosphofructokinase-regulated reaction. Why might ingesting high levels of fructose have deleterious physiological effects: phosphoglucomutase has a phosphorylated active site residue. The phosphoryl group is slowly lost by hydrolysis. Why is conversion of lactate into glucose in the liver important for the organism: lactic acid fermentation and alcoholic fermentation are oxidation-reduction reactions. The experiment consisted of incubating a small amount of 14c-labeled substrate (the pulse) with the yeast extract just long enough for each intermediate in the pathway to become labeled. The label is then chased through the pathway by the addition of excess unlabeled glucose. The chase effectively prevents any further entry of labeled glucose into the pathway.
