BIOL 200 Study Guide - Final Guide: Dna-Binding Domain, Homeosis, Gtpase
22 Jun 2016
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Tbp is important for all classes of transcription for optimal e ectiveness. H (helicase activity) + (phosphorylation of c terminal end via protein kinase) Tbp lacks transcriptional activators, it is one player in the mega tfiid complex all required for transcription. Basal recognition recognize bases through the major groove in dna. Changes the formation of the helix after binding (non-covalent) Reporter genes useful to indicate where transcription is happening (if they are downstream of a promoter) If you remove areas of the control region, you can a ect transcription some regions are required, some are not. Any entity that interacts with the probe, that also changes the mobility is most likely a dna binding protein you can gure out the exact sequence bound b the probe through electrophoresis. Gal4 binds to uas, activates downstream, reporter gene (lac z gene). N terminus is required to activate the uas. There is activation domain downstream on the c terminus.
