BIOL 200 Study Guide - Final Guide: Isomerase, Ern1, Peptide

• School: McGill University
• Department: Biology
• Course: BIOL 200
• Professor: Richard Roy

Document Summary

You can use arti cial ammoniacal-synthetase to create trna that interact with non- standard codons (get a stop codon to code for an amino acid) 23s (peptidyltransferase) binds the 2 amino acids together. (no protein requirement) Ef2-gtp is hydrolyzed to ef2-gdp and moves the trnas down 1 binding site (p to e, a to p) ratchet the trna in the e site is expelled once the ef1alpha-gtp is hydrolyzed. This continues until there is a stop codon bound by erf1. When the stop codon binds to a site, erf3-gtp binds to erf1. erf3-gtp hydrolyzes, cleaves o the amino acid chain. The whole ribosome then dissociates leaving just the 40s initiation complex. If you didn"t circularize the dna, the mana would be chewed up from the 3" end. eif4g binds to pabp1 to keep the mrna a circle. You can use centrifugation to determine when the complex is at di erent stages.