BIOL 200 Study Guide - Final Guide: Enzyme, Pseudoknot, Covalent Bond
Document Summary
Nucleotides phosphate group (negative charge) attached to sugar via thirster bond (pentose) sugar molecule linked to a nitrogenous base. Nucleotide > nucleoside + phosphate base (mono, di, tri) Agtc attached to it free phosphate at 5" carbon end free oh at the 3" carbon end. B dna - normal right handed helix major and minor groove (dna recognition protein) Proteins get to the base pairs through the grooves. A dna - wider helix (right handed) dry dna. Z dna - thin helix, tall (left handed) alternating purine - pyrimidine. Tm = point where half the molecules are denatured based on g-c content. As you increase temp, eventually the double stranded dna becomes denatured. If you lose the conformation, you can lose the function. Tertiary structure stabilized by hydrophobic interactions non polar side chains hydrogen bonds on polar side chains disulphide bonds on cysteine residues. Many polypeptides can have multiple motifs/domains ex. calcium binding domain, dna binding motif.