BIOL 200 Study Guide - Final Guide: Helicase, Deoxyribose, Dna Glycosylase

• School: McGill University
• Department: Biology
• Course: BIOL 200
• Professor: Richard Roy

Document Summary

Shine delargno sequence : shows where the rst aug is located (met) If1 + if2 (50s subunit) forming 70s initiation complex. Tf (termination factor) also called rf (release factors) Rf1 + rf2 mimic trna attracts rf3, clipping of covalent bond of trna and polypeptide. Primer sequence (only dna polymerization not rna polymerization) usually rna primer. Origin of replication is an at rich area. Rna pol (primase) adds the rna primer after dna helicase (large t antigen) unzips dna. Rpa attaches to keep the single stranded dna in optimal conformation. Dna polymerase epsilon (e) eukaryotes extends the primer sequence with dna. Pcna allows the complex to stay on the template. Polymerase alpha (a) extends the primer with dna (short dna sequence) Polymerase delta (d) replaces pol a and primase complex completes synthesis of okazaki fragment. When dna pol d hits another rna primer sequence: Ribonuclease h and fen1 displace rna primer of okazaki fragment.