Question

Determine the quantity of each chemical that you are going to use for preparing the buffer. Calculate the amount you will need to weigh out.

Stock Solutions to Prepare:
a.) 0.5 L of 0.2 M monobasic sodium phosphate (A)
b.) 0.1 L of 0.2 M dibasic sodium phosphate (B) (sometimes precipitation is observed over time so this should be made freshly)
c.) 100 mL of 1 M Tris base pH 8
d.) 250 mL of 4 M NaCl
e.) 20 mL of 4 M imidazole
f.) 10 mL of 0.5 M MgCl2

Estimate Buffer pH Adjustment
To calculate the amount of acid needed to adjust the pH of the Tris base (pKa = 8.3) to pH 8 use the Henderson-Hasselbalch Equation.