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29 Nov 2019

Use the Henderson Hasselbalch equation to calculate the volume in mls of a) Tris acid and b) Tris base to use to make 250 ml of a 0.01M Tris buffer, pH 8.0, from 0.01M stock solutions. Give your answer to one place of decimals. pKa Tris = 8.3.

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17 Dec 2019

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You wish to prepare 250. mL of a 0.300 M Tris buffer at room temperature (25 C) such that it has a pH of 7.80 at 10.0 C.
Starting with Tris base (F.W. 121.2, 99.9 % purity), answer the following questions regarding the preparation of this buffer. Refer to Table 2-1 in your coursepack for information regarding the temperature coefficient of Tris (âpH/âT).

-What mass of Tris is needed?

-To what pH (at room temperature, 25 C) would you adjust the buffer so that it had a pH of 7.80 at 10.0 C?

-Which would be the correct solution to use to adjust the pH?

-What volume of the 2 M solution identified above will be needed to reach the correct pH?

Buffers and pH

1.(12 pts) A stock solution of Tris -HCl buffer sits on the shelf (0.6 M, pH 8.35). You remove 80 mL of the stock solution, add 12 mL of 0.75M HCl and dilute to 1 L. The pKa of Tris-HCl is 8.20.

(a) How many millimoles of both forms of Tris -HCl are present after you added the acid?

(b) What is the final buffer concentration in mM?

(c)What is the final pH of your buffer?

Can you show all work to confirm the answers below:

(a)U = 19.1 mmoles, P = 28.9

(b)48mM

(c)pH = 8.02

Consider a 1.00 L buffer prepared by mixing 610 mg of Tris [tris(hydroxymethyl

aminomethane] (pKa = 8.07) in the free-base form (FW = 121.1 g/mol) and 4 mM HCl.

a) What is the pH of the buffer?

b) What would the pH be if we had added 2.8 mM of HCl instead of the amount of HCl in (a)?

c) What would the pH be if we had added 1.00 mL of 0.010 M NaOH to the original amounts in

(a)?

d) Could you use a Tris buffer system to prepare a buffer at pH 7.00? Why or why not?

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Use the Henderson Hasselbalch equation to calculate the volume in mls of a) Tris acid and b) Tris base to use to make 250 ml of a 0.01M Tris buffer, pH 8.0, from 0.01M stock solutions. Give your answer to one place of decimals. pKa Tris = 8.3.

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