Using structural formulas and resonance structures, explain why m-nitro substitution of chlorobenzene is much less favored than o-, p-nitro substitution. Flash column chromatography was performed on a mixture containing two fractions substances, and a total of ten fractions were collected. Analysis of the TLC gave the following results. Based on the TLC results, describe what substances are in each of the ten fractions. That is, which fractions contain only the more polar substance, which contain both substances? How is the separation of two substances using flash column chromatography likely to be affected if the solvent flow rate is greater than 2 in/min? Explain your reasoning. How is the separation of two substances using flash column chromatography likely to be affected if the silica gel stationary phase is disturbed while the column is being filled with solvent? Explain your reasoning.