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ANSC 3040 Quiz: Laboratory 1_Worksheet_Winter 2022

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chillandnetflix
19 Apr 2023
School
University of Guelph
Department
Animal Science
Course
ANSC 3040
Professor

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Document Summary

Determining the presence or concentration of hormones in body fluids. After completing this laboratory exercise, you should be able to: Understand the importance of knowing the concentration of hormones in biological samples for areas such as research, clinical applications, and herd management decisions. Explain, illustrate and execute an elisa and a lfia for quantification of hormones in biological samples. Hands-on activities: using elisa to detect the presence of pags in bovine plasma samples for pregnancy diagnosis in lactating cows. The elisa is a plate-based assay technique designed for detecting and quantifying antigens (peptides, proteins, lipids, etc ). In an elisa, an antigen must be immobilized to a solid surface and then complexed with an antibody that is linked to an enzyme. Detection is accomplished by assessing the conjugated enzyme activity via incubation with a substrate to produce a measureable product. The most crucial element of the detection is a highly specific antibody-antigen interaction.

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