CHM270 Study Guide - Midterm Guide: Salicylamide, Capillary Action, Phenacetin
Document Summary
Thin layered chromatography is often used to determine the purity of a compound. Tlc analysis, separation of components is achieved based on the differences in polarity in a sample. When a sample is applied to a chromatography sheet, the non-polar organic solvent, which is also known as the mobile phase, moves up the plate through capillary action away from the solvent. While the polar portion remains stationary at the applied point of origin. The plate used in the experiment is coated with fluorescent indicator to allow us visualize movement of the drugs under uv light. By being able to visualize this, we will be able to determine the rf value for each drug. The rf value is used to identify each component in the plate. The purpose of this experiment is to identify single components of a mixture of analgesics found in several pain killers and to identify unknown analgesic using thin-layer chromatography and to calculate the.
