BIOL 2114L Lecture 12: Indirect Enzyme Linked Immunosorbent Assay (ELISA) BIOL 2114 Lab - PhysioEx Exercise 12 Activity 3

Medical testing: enzyme linked immunosorbent assay (ELISA) test for presence of antigen or antibody

EXPERIMENTAL PROTOCOL

In this laboratory, you will test seven different patients using a direct ELISA. We have access to serum samples from the main characters of the TV show The Big Bang Theory. You will test each of them for the presence of ZIKA virus and HIV, as well as perform a pregnancy test, using known antigens/antibodies for those diseases/test. Students will work in pairs. Serum for each character of the Big Bang Theory has been incubated overnight at 4°C in the wells of an ELISA plate.

1) Interpretations: Summarize the interpretation of the findings about the results and the experiment

2) Future Scientific Directions: suggest future experiments using as base the results obtained in the lab

3) Create a question to the lab experiement approach.

5) Approach: Create a summary of the overall lab experiment. Hypothesis.

Medical testing: enzyme linked immunosorbent assay (ELISA) test for presence of antigen or antibody

EXPERIMENTAL PROTOCOL

In this laboratory, you will test seven different patients using a direct ELISA. We have access to serum samples from the main characters of the TV show The Big Bang Theory. You will test each of them for the presence of ZIKA virus and HIV, as well as perform a pregnancy test, using known antigens/antibodies for those diseases/test. Students will work in pairs. Serum for each character of the Big Bang Theory has been incubated overnight at 4°C in the wells of an ELISA plate. You are taking it from here.

1.) What basic principles of antibody-mediated immunity are utilized in an ELISA assay?

2.) What might have caused some positive results to be lighter in color than others?

3.) Why can some viruses not be targeted by antibodies for destruction? Is this a limitation for developing an ELISA-based protocol for detecting the disease?

4.) Postulate a hypothesis on how the characters infected with Zika contracted the disease (in time and space); consider that two of them live in the same apartment and the others visit them frequently.

SYMBIOTS LAB different from above ^

1. If you give a high temperature and high light treatment to Symbiodinium linchaea and Symbiodinium minutum, what results do you expect after 3 weeks of treatment?

ELISA lab question: When you added primary antibody to thewells, what happened if your sample contained the antigen? What ifit did not contain the antigen?