MCD BIO CM156 Lecture Notes - Lecture 18: In Situ Hybridization, Petri Dish, Capillary Electrophoresis
8 Mar 2020
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Techniques applied to molecular diagnosis: southern blot, allele-specific probe hybridization, dna sequencing (sanger & ngs, polymerase chain reaction, rt-pcr, mutation scanning: sscp, dgge, dhplc, capillary electrophoresis, protein truncation test, real-time pcr, invader assay, in situ hybridization, microarrays. Genetic application has raised a lot of ethical issues. A lot of techniques now are pcr and sequencing based. Father donates sperm, woman must go through oocytes (harder to get) Put both on a petri dish and let the embryos grow to the 8 cell stage. Check the pluripotent embryos and do single cell pcr on it. If you see no mutation in the embryo, implant it back into the mother"s womb. Paper shows you can have the patient do a mouthwash to obtain the dna. Can use hair, tears, anywhere that can be analyzed by pcr. Genetic application has raised problems of ethical abuse. Prenatal diagnosis (would disappear if abortion was illegal)
