BIO 201 Lecture Notes - Lecture 9: Intermediate Filament, 2-Mercaptoethanol, Polyacrylamide
6 Sep 2018
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The cytoskeleton: analyze using sds-page, microtubules, actin. Smallest one is microfilaments, then intermediate filaments, then microtubules. Characterization of cytoskeletal proteins using sds polyacrylamide gel electrophoresis (sds-page) Separate base on size, then denature protein by boiling it, but it cannot permanently denature the protei(cid:374). Sds-page you are, the easier to get through the pores. Anode are positive, and protein are attracted more as it stacks up to cathode which is negative. Discovery that the three types of mts have a common structural basis. Alpha and beta tubulin subunits form heterodimers. Polymers only in the presence of gtp: plus (+) end: polymerization favorable, minus (-) end: polymerization unfavorable. Mts are in a dynamic equilibrium of polymerization and depolymerization at all times. This is called dynamic instability or tread milling. Is microtubule organizing center (mtoc) in plants and animals. Made from centriole (mts) and other proteins. Mt arrangement: minus (-) ends towards the nucleus, plus (+) ends toward the plasma membrane.
