BSC 315 Lecture Notes - Lecture 12: Phosphodiester Bond, Dna Ligase, Telomere

• School: University of Alabama
• Department: Biological Sciences
• Course: BSC 315
• Professor: Edwin Stephenson

Document Summary

Copying of dsdna molecule into 2 new identical molecules. Occurs in interphase in preparation for mitosis or meiosis i. Semiconservative dna replication- each old strand serves as the template to synthesize a new strand. Both strands in one entire molecule would be old, & both strands in the copy new. Was noted & predicted by the watson-crick model of dna structure. Each strand serves as the template to make a new complementary strand. The nucleotide next to be added is a nucleotide triphosphate (3 (cid:1) (cid:1) (cid:1) (cid:1) (cid:1) phosphates) (cid:1) Bond between 1st and 2nd phosphate is broken, nucleotide (w/ 1 phosphate) is added (cid:1) (cid:1) (cid:1) (cid:1) (cid:1) Dna is synthesized 1 nucleotide at a time. The new strand is synthesized from 5" to 3" only. Replication begins at 1 or more origins, each called an origin of replication. A small region is pulled apart into single strands, a replication bubble (cid:1) (cid:1)