BSC 315 Lecture Notes - Lecture 9: Okazaki Fragments, Dna Ligase, Sister Chromatids
16 Oct 2016
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This copying must precede the transmission of chromosomes from one generation to the next. Also the basis for chromosome duplication prior to mitosis. Overview: complementary base pairing ensures semiconservative replication a. b. c. d. The two strands of the watson and crick double helix model of dna unwinds and separates to leave two exposed single template strands. These newly formed replicate strands are complementary (by base pairs) i. ii. This process yields the formation of two daughter strands of dna, each with one parental strand i. This occurs post-enzymatic polymerization of nucleotides via phosphodiester linkages. Experiments with heavy nitrogen verify semiconservative replication a. b. The premise was to grow cultures of escherichia coli in a medium of a 15n heavy isotope to incorporate this isotope into the nitrogenous bases of dna. Once they were incorporated, these bacteria were transferred to a medium of 14n isotope.
