BIO 204 Lecture Notes - Lecture 11: Organophosphate, Insecticide, Spectrophotometry
15 Dec 2018
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Document Summary
Nerves communicate with muscle cells through ach. Ach is released from the presynaptic cell. Binds to receptor, leading to depolarization muscle twitch. Organophosphate insecticide binds to block ach from binding to the active site. Not harmful to humans because of size and don"t breathe through skin. Used to kill off mosquitoes to prevent malaria created survival pressure on mosquitoes and they adapted to be insecticide resistant. Hydrophobic side chains tend to gather together near the center of the protein, away from the water. Hydrophilic side chains tend to be on outside. Positive and negative side chains tend to pair up. Cysteines come together to form disulfide bonds and will be closer together. Activity 3a: blank the spectrophotometer with the buffer and dntb solution without cysteine, measure the absorbance values of the varying concentrations of cysteine, graph these values with a trend line and r2 value.
