BIOL 039 Lecture Notes - Lecture 25: Transposase, Moe Williams, Transposable Element
Document Summary
Structure of the ac/ds transposons in maize. Structure of jumping genes in maize reflects general structure of tes. Both ac and ds are flanked by 11-bp ir that are essential for transposition. The ac element encodes a transposase that is responsible for excision and transposition. Ds elements are structurally hetero-geneous, internal sequences encoding for transposase are missing. This is why ds elements cannot transpose themselves; they require the presence of the ac transposon, in the cell to provide the transposase. The ac element encodes a single protein, the transposase. The transposase can bind to irs of ac and ds elements, as both are members of the same transposon family; transposases can move elements of their own family but not others as they do not recognize other irs. Upon binding, transposase excises the element, cleaves the target site and. Transposable elements in bacteria promote insertion at new location. Bacterial transposons move within a chromosome and between a chromosome and plasmids.