BIOL 039 Lecture Notes - Lecture 26: P Element, Moe Williams, Transposable Element
Document Summary
Conservative (or cut-and-paste) and replicative (or copy-and-paste) Conservative transposition excises a composite transposon (e. g. , tn10) from one location and inserts it into a new one. The process moves transposable elements around the genome but does not increase in the number of te per genome. Replicative transposition copies a simple transposon (e. g. , tn3) and inserts a copy into a new location. This process increases the number of te per genome. As the name implies, the replicative transposition involves replication of transposons. During transposition between two plasmids, transposase facilitates formation of a cointegrate a temporary fusion of two plasmids. A recombination-like process between the two transposons. Resolves the cointegrate, and leaves both plasmids with a copy of the element. A donor plasmid carrying tn3 transposon pairs with a target plasmid carrying a target sequence. Transposase creates staggered cuts in dna adjacent to tn3 and at the target sequence. Strands crossover forming a cointegrate; dna synthesis fills gaps.
