CGS NS 201 Lecture Notes - Lecture 3: Tas2R38, Zygosity
17 Oct 2017
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Listed above are two alleles for the tas2r38 gene (with both strands of dna shown for each allele). The forward and reverse primers are also shown. Recall the base pairing rules for this question. The c and g are the differences: given the following strands of dna, which will be cut by haeiii, 3"-gcaggcaccggactgt-5", 3"-gattctatgtagcgat-5", 5"-agtccgggcatatgga-3", 3"-gcaggcacccgactgt-5". Tb 5" prime: 1st fragment 46, 2nd is 176. Ta 3 prime: 1st fragment 90, 2nd is 130. We would expect to see 2 fragments for allele ta and 4 for tb. Heterozygous would 1 from ta and tb, in total it will be 3. After running the gel, it will separate the fragments by size. The fragments will be as long as the bases are. The gel could be darker but you would only see one band if they are the same size.
