BIOL 311 Lecture Notes - Lecture 9: Short Hairpin Rna, Hela, Prokaryote
23 Sep 2016
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1970s - begin to take them seriously! they are important in studying cell biology. Hartwell - yeast: temperature sensitive mutant! eukaryote haploid, diploid easy to grow temperature sensitive mutant how do we do this? we can grow them at 23 c - important temp b/c permissive temp. 36 c is a non permissive temperature complementation analysis - determines whether two recessive alleles/mutations are on the same gene. Look in the textbook for this (said this many times - important look at. Principles and concepts: restriction nucleases - molecular biology began with restriction nucleases (aka en- zymes aka endonuclease) Restriction nucleases - discovered originally in bacteria (look in book) used to cleave bacteriophage dna. Rns are non-random - when choose one; they cleave at specified sites. Probably around > 600 restriction nucleases available: gene splicing and transfection, nucleic acid hybridization. A very simple concept - we"ve talked about it before.
