BIOL 311 Lecture Notes - Lecture 7: Reverse Transcriptase, Medical Jurisprudence, Personalized Medicine
Document Summary
A group of processes used to manipulate, analyze, and express nucleotides to better understand cell function. Proteomics: study of entire proteome (all of your proteins) Epigenetics: modifications to dna that don"t involve a change in base sequence. Secretome: what cells secrete (ex. proteins, dna, etc. ) Chemical carcinogens - generate cell lines/strains: ethyl methane sulfonate takes g-c pair and transforms into a-t pair. Crispr-cas9: edits genes (specific enough to remove one base pair) Mutant mice: knock in/knock out mice ~ crispr can control gene expression. Complementation analysis: determine if two recessive mutations are on the same gene. Principles and concepts: restriction nucleus (aka enzymes, endonuclease, 1970s: sds gel electrophoreses. Problem: dna too long: restriction nucleases. Cleave at specified sites: > 600 rns available, gene splicing and transfection, nucleic acid hybridization, 1968 britten and kohne. Can be used as a tool: harness the above for dna engineering and expression of certain proteins (ex. biologics)
