BIOL3190 Lecture Notes - Lecture 27: Chromosome, Exon, Transcriptome
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Use hydroxyl-less nucleotides called dideoxynucleotides to truncate the chain and then you look at the one cell will express a unique set of genes same genome different transcriptome. (cid:455)ou add a (cid:374)e(cid:449) (cid:374)u(cid:272)leotide that (cid:455)ou (cid:272)a(cid:374)"t e(cid:454)te(cid:374)d and there is a color fluorophore that detects that. Both have to be reversible but you can add to it. The fa(cid:272)t that the 3" h(cid:455)d(cid:396)o(cid:454)(cid:455)l is (cid:271)lo(cid:272)ked is (cid:271)e(cid:272)ause o(cid:454)(cid:455)ge(cid:374) is hooked up to so(cid:373)ethi(cid:374)g else. Sanger sequencing length of the chain to deduce what base was added last. Slide with a bunch of amplified genome on it and a little pump puts a new fluid into the chamber so that it will have fluoresce. Then you put in a new chemical that can cleave off the fluorophore. Idea of studying sequences in context of entire genome. Transcriptome all of the rnas that are made by a given cell type.
