CHEM 3050 Lecture Notes - Lecture 34: Turnover Number, Enzyme Kinetics, Hyperbola
3 May 2018
School
Department
Course
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CHEM 3050
Lecture 34
Michaelis-Menten Equation
Equation for a hyperbola:
Steady State Kinetics
- [S] Decreases, and [P] increases at a constant rate
over time
- [E] (Free enzyme) decreases at first and then it
stabilizes
- [ES] increases initially then does not change over
time
o This occurs when [S] >> [E]
- Most of the enzymes are bound to substrate
o E saturated with S
KNOW HOW TO USE VO, LINEWEAVER-BURK,
COMPETITIVE, NONCOMEPETITIVE/MIXED,
UNCOMPETITIVE INHIBITION EQUATIONS AND
∆ψ (WATCH THE UNITS!!).
Initial Velocity vs. [S]
- Catalytic rate constant: Kcat the turnover number
of enzyme
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