Biology 1202B Lecture Notes - Lecture 8: Base Pair, Southern Blot, Restriction Fragment Length Polymorphism
15 Jun 2016
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Document Summary
Dna technologies rely on enzymes: often modified perfom better. Bacterial defence mechanism: methylate re sites in own genome, re digest invading dna. Cut dna at specific sequence: 4-8 base pair recognition, usually symmetrical. Separation based on size: largest dna near negative end, smallest dna near positive end. Intercalates between bases: flat molecule, uv fluorescent, non- specific. Blotting is the transfer of dna from the gel to a charged membrane. Allows you to identify a specific dna sequence: use a probe complimentary to gene of interest. Probe is complementary to gene of interest: labelled with a marker. Dna differences which can be detected by length of fragements after re digestion: may be used to detect disease, inherited in a co-dominant fashion. Mutationin the b-globin gene: causes sickle shaped cells, cells stick and accumulate at branching points in circulatory system, co-dominant trait. If only 1 copy is mutated both normal and sickle cells.
