Biology 1201A Lecture Notes - Lecture 18: Agarose Gel Electrophoresis, Zygosity, Restriction Fragment Length Polymorphism
12 Jan 2016
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1978 nobel prize in physiology or medicine. Negatively charged dna moves towards positive electrode. Blotting is the transfer of dna from the gel to a charged membrane. Allows you to identify a specific dna sequence. Use a probe complimentary to gene of interest. Dna differences which can be detected by length of fragments after re digestion. Cells stick and accumulate at branching points in circulatory system. Co-dominant trait (if only 1 copy is mutated both normal and sickle cells) The co-dominant trait phenotype confers resistance to malaria. The mutation more prevalent in people who"s ancestors lived in region where malaria is/was common. Sickle cell disease is detected by a rflp. The sickle cell mutation changes an re site. Mstii cuts 3 times in normal dna. Mstii cuts 2 times in the mutated dna. The probe is designed to discern between the two alleles. Probe with antibody specific to protein of interest. Can also use them to cut and paste dna.
