Biology 1201A Lecture Notes - Lecture 3: Ap Site, Amine, Deoxyribose

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emeraldwallaby169

27 Mar 2016

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The amount of dna is one genome (i. e. one complete set of chromosomes) Genome (c value)= the amount of dna in a complete set of chromosomes. The c value is a measure of size count the number of chromosomes. Number of sets of chromosomes in the nucleus of a cell. Karyotype= artificial replication of genome in an organism. Chromosomes never line up this way, we take pictures of them during metaphase, stain them and line them up. In this picture, technically 46 chromosomes but there are only. Haploid refers to one set of unique chromosomes. C value is the amount of dna in an unreplicated haploid cell. C-value characterizes species all humans have the same c-value. Actual amount of dna in a person changes throughout the cell cycle. Most of your genome is not genes. 8%- centromeres important structural elements that have functions but don"t code for genes.

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The bacterium E. coli, which is a major model system for the study of genes and DNA, has a singular circular chromosome of about 4x106 bp. In contrast, human diploid cells have __1__ chromosomes, of __2__ different types (donât forget that the X Â¹ Y!). The total amount of DNA found in a haploid cell such as a sperm or egg is called the organismâs âC-valueâ; the human C-value is __3__.

Also in contrast to the E. coli circular chromosome is the fact that eukaryotic chromosomes are not circular, but instead are __4__. Eukaryotic chromosomes are complexed heavily with protein to form a DNA-protein complex generically called __5__. The proteins found can be considered to fall into two general classes: __6__ and __7__. The first class is made up of 5 major proteins called __8__, __9__, __10__, __11__, and __12__. On a weight to weight basis, these proteins make up about __13__% of the total mass of a chromosome. Since these proteins must associate relatively non-specifically with DNA, they are rich in the basic amino acids __13__ and __14__, which gives the proteins a net __15__ charge.

One goal of packaging the DNA into chromatin is to compact the genome so it will fit into a nucleus. The basic unit of chromatin structure is the __16__, which has __17__ base pairs of DNA wrapped around a âcore particleâ composed of __18__ copies each of the core histones. Each nucleosome is separated by a stretch of âlinkerâ DNA so that the repeating unit is approximately 200 bp. Lets do a little arithmetic: If we consider that the nucleosome is approximately __19__ nm in diameter, that there is about 200 bp in this structure, and that 200 bp of DNA would normally occupy __20__ nm if stretched out straight (recall that each base is spaced about 0.34 nm from the next), we can see that the nucleosome alone allows for a __21__-fold compaction ratio.

The nucleosome structure is further compacted by histone __22__, which is not a part of the core particle. Instead, this histone __23__ (does what?) to cause the nucleosomal 10 nm fiber to condense into a higher-order structure referred to as the __24__ nm fiber. In the nucleus, chromosomes do not just float around freely: they are tethered to a proteinaceous structure called the __25__ at specific DNA regions called âSARsâ, which stands for __26__. SARs are rich in topoisomerases, to allow the winding and unwinding of the DNA that accompanies replication and transcription to occur, despite this constraint.

The 30 nm fiber discussed above is the form in which transcriptionally active DNA is believed to exist in the nucleus; however, further condensation leads to a highly condensed inactive form called __27__; in this terminology, the active form is referred to as __28__. The inactive condensed form can be divided into two types: one type is essentially invariant between cell types, and since it is âon all t timeâ, it is referred to as __29__. The other type comes and goes, depending on the expression of the genes present in that region of the chromosome; it is referred to as __30__.

Eukaryotic chromosomes carry the genes, of course, but also have other functional regions. One such region is the place where the spindle apparatus attaches during mitosis and meiosis; this region of the chromosome is called the __31__. Another important part of the linear eukaryotic chromosome is the ends; these are called __32__. In humans, the end of each chromosome is composed of many repeats of the short sequence __33__. One more type of functional region on each chromosome is the place where DNA replication starts; in humans, each chromosome has several of these __34__. Thus, eukaryotic chromosomes carry several different types of functional sequences, which together allow for their __35__ during S phase, __36__ during mitosis and meiosis, and the __37__ of their genes

20. All living things must maintain a constantinternal environment to function properly through the process of__. (Points : 2)

metamorphosis
alkalosis
homeostasis
cryogenesis

Question 21.21. Glucose, fructose, sucrose,starch and glycogen are all examples of ___. (Points : 2)

proteins
carbohydrates
lipids
nucleic acids

Question 22.22. Some insects can "walk onwater" because __. (Points : 2)

water has a high surfacetension
water is adhesive
water istransparent
water expands when itfreezes

Question 23.23. To overcome an energy barrierbetween reactants and products in a chemical reaction, energy mustbe provided to get the reaction started. This energy is called ___energy. (Points : 2)

potential
reaction
initiation
activation

Question 24.24. Where in the cell doesglycolysis takes place? (Points : 2)

lysosomes
cytoplasm
mitochondria
nucleus

Question 25.25. Photosynthesis uses sunlightto convert water and carbon dioxide into __. (Points : 2)

carbon dioxide andwater
oxygen and sugars
proteins and sugars
carbon dioxide andoxygen

Question 26.26. The _____ of photosynthesisare the _____ of cellular respiration. (Points : 2)

electrons, protons
pigments, products
products, reactants
elements, atoms

Question 27.27. The energy currency of thecell is __. (Points : 2)

glucose
ATP
starch
glycogen

Question 28.28. When blood glucose levelsdecrease (as between meals), what reserves are tapped first?(Points : 2)

glycogen
fats
steroids
amino acids

Question 29.29. When glucose is used as anenergy source, the largest amount of ATP is produced by the ____portion of the entire respiratory process. (Points : 2)

glycolytic
Kreb's cycle
acetyl-CoAformation
electron transportchain

Question 30.30. Pyruvic acid can be regardedas the end product of ____. (Points : 2)

glycolysis
fermentation
the Kreb cycle
the electron transportchain

Question 31.31. How can one increase the rateof a chemical reaction? (Points : 2)

Increase the activationenergy.
Cool the reactants.
Decrease the pH.
Add an enzyme(catalyst).

Question 32.32. A prokaryote has ___. (Points: 2)

membrane-boundorganelles
nucleus
mitochondria
a cell membrane

Question 33.33. Unlike animal cells, plantcells have __. (Points : 2)

cell membranes
cell walls
chromosomes
mitochondria

Question 34.34. Which of the followingrequires energy to move molecules across the cell membrane? (Points: 2)

diffusion
osmosis
active transport
passive transport

Question 35.35. The ___ is the site of proteinsynthesis. (Points : 2)

nucleus
ribosome
mitochondrion
cytoskeleton

Question 36.36. Diffusion of water through asemipermeable membrane is called __. (Points : 2)

osmosis
diffusion
active transport
passive transport

Question 37.37. Which component of a cellmembrane is likely to function as a transporter? (Points : 2)

cholesterol
lipids
proteins
carbohydrates

Question 38.38. If potato slice is placed indistilled water, the cells would most likely __. (Points : 2)

lose water and becomelimp
neither take up water orlose water
take up water andswell
transport water into thecell by active transport.

Question 39.39. The chloroplast functions in___. (Points : 2)

protein synthesis
lipid storage
DNA replication
photosynthesis

Question 40.40. The fluid mosaic modelpresents the modern view of ___. (Points : 2)

the cell membranestructure
chromosome movementduring mitosis
protein synthesis
ATP production

Question 41.41. During which stage of mitosisdo nucleoli reappear and the nuclear membrane reforms? (Points :2)

prophase
metaphase
anaphase
telophase

Question 42.42. During which stage of mitosisdo chromatids separate to form two sets of daughter chromosomes?(Points : 2)

prophase
metaphase
anaphase
telophase

Question 43.43. Which of the following is thelongest stage of mitosis? (Points : 2)

prophase
metaphase
anaphase
telophase

Question 44.44. When during the cell cycle isDNA replicated? (Points : 2)

metaphase
Synthesis phase (S)
G1
cytokinesis

Question 45.45. A cell with 10 chromosomesundergoes mitosis and cell division. How many daughter cells areproduced and what number of chromosomes do they have? (Points :2)

4 daughter cells, 20chromosomes each
4 daughter cells, 5chromosomes each
2 daughter cells, 10chromosomes each
2 daughter cells, 5chromosomes each

Question 46.46. Maize (corn) has a haploidnumber of 10. How many chromosomes are in a zygote of maize?(Points : 2)

5
10
20
Not enough informationprovided

Question 47.47. Crossing over is one of themost important events in meiosis because __. (Points : 2)

homologous chromosomesare separated into different daughter cells
the number of chromosomesallotted to each daughter cell must be halved
sister chromatids must beseparated into different daughter cells
it produces new arrays ofalleles on chromosomes and contributes to genetic diversity

Question 48.48. While viewing stained onionroot tip cells you notice that distinct chromosomes cannot beobserved in most of the cells. This is because most of the cells___. (Points : 2)

were killed whilepreparing the slide
are in Interphase
are in metaphase
are undergoingMeiosis

Question 49.49. Each offspring cell producedby binary fission contains __. (Points : 2)

half the chromosomes ofthe original cell
twice as many chromosomesas the original cell
an independent assortmentof the original cell's chromosomes
an identical copy of theoriginal cell's chromosome.

Question 50.50. A DNA strand has the sequenceA-C-A-G-C-C-G-T-A. What would be its complementary strand? (Points: 2)

G-T-G-A-T-T-A-C-G
A-C-A-G-C-C-G-T-A
T-G-T-C-G-G-C-A-T
U-G-U-C-G-G-C-A-U

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Question 1

The process by which RNA is synthesized using DNA as a template is â¦

Select one:

a. replication

b. transcription

c. translation

d. inspiration

Question 2

Which of the following occurs during meiosis but not mitosis?

Select one:

a. DNA condenses into chromosomes

b. nuclear envelop disappears

c. homologous chromosomes separate

d. sister chromatids separate

Question 3

Which part of the animal life cycle is haploid?

Select one:

a. gamete

b. zygote

c. sporophyte

d. spore

Question 4

Single celled eukaryotic organisms are â¦

Select one:

a. viruses

b. bacterial

c. archaea

d. protists

Question 5

Which type of cellular division results in the formation of diploid cells from a diploid parent cell?

Select one:

a. mitosis

b. meiosis

c. interphase

d. A and B but not C

e. all of the above

Question 6

A sequence of DNA that codes for a single protein is a â¦

Select one:

a. gene

b. ribosome

c. mRNA

d. none of the above

Question 7

Which of the following is characteristic of animals?

Select one:

a. heterotrophic

b. sexual reproduction

c. embryonic development

d. all of the above

Question 8

All genes have only two possible alleles.

Select one:

True

False

Question 9

The presence of membrane-bound organelles is characteristic of â¦

Select one:

a. prokaryotes

b. viruses

c. bacteria

d. eukaryotes

Question 10

What are the building blocks of proteins?

Select one:

a. fatty acids

b. amino acids

c. nucleic acids

d. all of the above

Question 11

During which phase of the cell cycle does DNA replication occur?

Select one:

a. Mitosis

b. Interphase

c. Meiosis

d. Replicosis

Question 12

Which of the following characteristic is shared among fungi and animals?

Select one:

a. autotrophic

b. heterotrophic

c. cell walls

d. absorb nutrients

Question 13

A error in DNA replication that goes unrepaired is a â¦

Select one:

a. chromosome

b. chromatid

c. mutation

d. none of the above

Question 14

Alternate forms of a gene are called ________.

Select one:

a. chromatids

b. chromosomes

c. ribosomal subunits

d. alleles

Question 15

The physical expression of a genotype is the phenotype.

Select one:

True

False

Question 16

In complete dominance, a heterozygous genotype results in â¦

Select one:

a. a recessive phenotype

b. a dominant phenotype

c. a third and unique phenotype

d. none of the above

Question 17

Which theory explains the evolution of eukaryotes from prokaryotes?

Select one:

a. phagocytosis

b. endosymbiosis

c. pinocytosis

d. none of the above

Question 18

Which scientist experimented with plants to describe patterns of inheritance?

Select one:

a. Mendel

b. Chase

c. Einstein

d. Watson

Question 19

Which cellular organelle contains aerobic cellular respiration?

Select one:

a. chloroplasts

b. mitochondria

c. nucleus

d. lysosome

Question 20

Which of the following statements is false?

Select one:

a. Haploid gametes are formed by meiosis

b. Mitosis forms genetically identical daughter cells

c. Mitosis results in the formation of four daughter cells from a single parent cell

d. Mitosis forms diploid daughter cells from a diploid parent cell

Question 21

The first forms of life on Earth were ________.

Select one:

a. plants

b. microorganisms

c. birds

d. dinosaurs

Question 22

Which of the following is used to separate chromosomes during nuclear division?

Select one:

a. cytoskeleton

b. microtubules

c. actin and myosin

d. amoeboid movement

e. all of the above

Question 23

Which generation is most dominant in more primitive plants?

Select one:

a. bryophyte

b. sporophyte

c. gametophyte

d. androphyte

Question 24

Which of the following is characteristic of plants?

Select one:

a. autotrophic

b. contain chloroplasts

c. multicellular

d. all of the above

Question 25

During which phase of mitosis do chromosome line up along the middle of the cell?

Select one:

a. prophase

b. metaphase

c. anaphase

d. telophase

Question 26

During which phase of mitosis does DNA condense into distinct chromosomes?

Select one:

a. prophase

b. metaphase

c. anaphase

d. telophase

Question 27

The process by which DNA is used as a template to make new DNA is â¦

Select one:

a. translation

b. replication

c. transcription

d. allocation

Question 28

Which of the following is characteristic of plant but not animal cells?

Select one:

a. chloroplasts

b. water vacuole

c. cell wall

d. all of the above

Question 29

Which meiotic division is most like mitosis?

Select one:

a. Meiosis I

b. Meiosis II

c. Meiosis III

d. Meiosis IV

Question 30

Which enzyme catalyzes replication?

Select one:

a. DNA polymerase

b. RNA polymerase

c. Ribosome

d. all of the above

Biology 1201A Lecture Notes - Lecture 3: Ap Site, Amine, Deoxyribose

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