Biology 1201A Lecture Notes - Lecture 4: Nitrogenous Base, Chromosome, Sister Chromatids

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30 Aug 2015

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Biology lecture notes lecture: september 22th, 2014: amount of dna might not have to do complexity, genome: amount of dna in a complete set of chromosome. Genome can be measured by counting nitrogen bases, or it can be measured in mass. Number of sets of chromosomes in the nucleus of a cell. Human : n=23, 46 separate double stranded dna. C value) genome: the amount of dna in a haploid cell (one chromosome in each pair) Humans are diploid (2n=46), 2 of every chromosomes. C values define species, as well as karyotypes of chromosomes. Dna amounts changes throughout the cell cycle (multiplying). Also, centromeres: dna coding where the microtubules attach to sister chromatids. (8 percent with other stuff) Introns: dna sequences that are removed from the dna. Most junks: transposons, dead genes (not functioning), viruses. Dna composed of nucleotides, coding for amino acids. (come in 20 different forms ) depending on the sequence of the nucleotides.

Related Questions

The bacterium E. coli, which is a major model system for the study of genes and DNA, has a singular circular chromosome of about 4x106 bp. In contrast, human diploid cells have __1__ chromosomes, of __2__ different types (donât forget that the X Â¹ Y!). The total amount of DNA found in a haploid cell such as a sperm or egg is called the organismâs âC-valueâ; the human C-value is __3__.

Also in contrast to the E. coli circular chromosome is the fact that eukaryotic chromosomes are not circular, but instead are __4__. Eukaryotic chromosomes are complexed heavily with protein to form a DNA-protein complex generically called __5__. The proteins found can be considered to fall into two general classes: __6__ and __7__. The first class is made up of 5 major proteins called __8__, __9__, __10__, __11__, and __12__. On a weight to weight basis, these proteins make up about __13__% of the total mass of a chromosome. Since these proteins must associate relatively non-specifically with DNA, they are rich in the basic amino acids __13__ and __14__, which gives the proteins a net __15__ charge.

One goal of packaging the DNA into chromatin is to compact the genome so it will fit into a nucleus. The basic unit of chromatin structure is the __16__, which has __17__ base pairs of DNA wrapped around a âcore particleâ composed of __18__ copies each of the core histones. Each nucleosome is separated by a stretch of âlinkerâ DNA so that the repeating unit is approximately 200 bp. Lets do a little arithmetic: If we consider that the nucleosome is approximately __19__ nm in diameter, that there is about 200 bp in this structure, and that 200 bp of DNA would normally occupy __20__ nm if stretched out straight (recall that each base is spaced about 0.34 nm from the next), we can see that the nucleosome alone allows for a __21__-fold compaction ratio.

The nucleosome structure is further compacted by histone __22__, which is not a part of the core particle. Instead, this histone __23__ (does what?) to cause the nucleosomal 10 nm fiber to condense into a higher-order structure referred to as the __24__ nm fiber. In the nucleus, chromosomes do not just float around freely: they are tethered to a proteinaceous structure called the __25__ at specific DNA regions called âSARsâ, which stands for __26__. SARs are rich in topoisomerases, to allow the winding and unwinding of the DNA that accompanies replication and transcription to occur, despite this constraint.

The 30 nm fiber discussed above is the form in which transcriptionally active DNA is believed to exist in the nucleus; however, further condensation leads to a highly condensed inactive form called __27__; in this terminology, the active form is referred to as __28__. The inactive condensed form can be divided into two types: one type is essentially invariant between cell types, and since it is âon all t timeâ, it is referred to as __29__. The other type comes and goes, depending on the expression of the genes present in that region of the chromosome; it is referred to as __30__.

Eukaryotic chromosomes carry the genes, of course, but also have other functional regions. One such region is the place where the spindle apparatus attaches during mitosis and meiosis; this region of the chromosome is called the __31__. Another important part of the linear eukaryotic chromosome is the ends; these are called __32__. In humans, the end of each chromosome is composed of many repeats of the short sequence __33__. One more type of functional region on each chromosome is the place where DNA replication starts; in humans, each chromosome has several of these __34__. Thus, eukaryotic chromosomes carry several different types of functional sequences, which together allow for their __35__ during S phase, __36__ during mitosis and meiosis, and the __37__ of their genes

M2A1 Worksheet: Mitosis and Meiosis

A dog is a diploid organisms and has 39 pairs of chromosomes (2n = 78), where n = 39 chromosomes from each parent. Use this example to answer the following question regarding chromosomal number.

INTERPHASE

How many chromosomes are present in the somatic cell?

What do they look like?

What are they âdoingâ in G1 of this phase?

What is happening in the S phase?

What is happening in the G2 phase?

What follows G2 phase in a somatic cell?

What follows G2 phase in a gamete cell?

MITOSIS

Number of chromosomes in the parent cell.

Number of chromatids in the parent cell.

Number of chromosomes in the daughter cell.

Number of chromatids in the daughter cell.

How many daughter cells are produced?

Are the daughter cells haploid or diploid?

How many cell divisions take place?

In what type of cell does this division occur?

Meiosis 1:

Number of chromosomes in the parent cell.

Number of chromatids in the parent cell.

Number of chromosomes in each Meiosis I daughter cell.

Number of chromatids in each Meiosis I daughter cell.

Are the daughter cells haploid or diploid at the end of Meiosis 1?

Meiosis II:

Number of chromosomes in the âparentâ cell (which is a daughter cell from Meiosis I).

Number of chromatids in the âparentâ cell.

Number of chromosomes in each Meiosis II daughter cell.

Number of chromatids in each Meiosis II daughter cell.

Are the daughter cells haploid or diploid at the end of Meiosis II?

Which phase or phases of Meiosis account for the genetic variability among the daughter cells? Howâ¦ what happens in this phase to create the diversity?

Comparing Mitosis and Meiosisâ¦..

How many daughter cells result from Mitosis? Are they diploid or haploid?

How many daughter cells result from Meiosis? Are they diploid or Haploid?

Cell structures and function: Match each organelle to its function:

Organelle Function

Lysosome 1. Lipid synthesis

Rough ER 2. Houses DNA

Nucleus 3. Energy extraction

Smooth ER 4. Dismantles debris

Golgi apparatus 5. detoxification

Mitochondria 6. Protein synthesis

Peroxisome 7. Processes secretions

What is the function of checkpoint proteins in the cell cycle?

How can all of a personâs cells contain exactly the same genetic material, yet specialize as bone cells, nerve cells, muscle cells and connective tissues?

What are the 3 primary germ layers?

Briefly, in one or two sentences, distinguish between

a bacterial cell and a eukaryotic cell

interphase and mitosis

mitosis and apoptosis

an autosome and a sex chromosome

homologous pair of chromosomes and a sister chromatid

centromere and centriole

chromosome and chromatid

monozygotic and dizygotic twins

allele and gene

13. The arms of a human and the wing of a bat are _________________ structures, composed of the same bones that have been modified for different functions.

analogous

vestigial

c.	homologous

d.	convergent

14. Xeroderma pigmentosum is an autosomal recessive genetic disorder. People who are homozygous for the disease-causing allele have skin that is damaged by exposure to ultraviolet radiation; this can lead to skin cancer at an early age. The mutation that causes this disorder is in a gene that functions:

a.	to repair DNA by excising (removing) nucleotides damaged by UV light.

b.	in lysosomes, to break down lipids that otherwise accumulate in the nervous system.

c.	to produce the UV blocking pigment melanin in the skin cells

d.	in red blood cells to carry oxygen to the skin.

15. The Amish are a religious sect descended from a small group of colonists who came to the United States over 200 years ago. Their religious beliefs have kept them isolated from society. The Amish have a much higher than average incidence of both polydactyly (extra fingers and toes) and dwarfism in their communities. This is most likely explained by:

a.	inheritance of acquired characters.

b.	the founder effect.

c.	disruptive selection.

d.	natural selection.

16. In the structure of DNA that Watson and Crick proposed, the sides of the "ladder" of the DNA molecule are always the same distance apart, making the molecule a constant width. This occurs because when the bases hydrogen bond to form the rungs of the "ladder", a:

a.	purine always pairs with a pyrimidine.

b.	purine always pairs with a purine.

c.	pyrimidine always pairs with a pyrmidine.

d.	The structure of the double helix is not determined by how the bases pair.

17. The RNA transcript of DNA that travels to the cytoplasm, carrying the instructions to make a protein, is called:

rRNA.

tRNA.

mRNA.

d.	RNA polymerase.

18. When DNA is replicated, the error rate is approximately one error for every 10,000 nucleotides copied. However, that error rate is reduced to only 1 error for every 1 billion nucleotides. The DNA sequence is "corrected" by:

a.	repeating S phase to see if the error rate is lower the second time around.

b.	enzymes that proofread the DNA and repair errors.

c.	messenger RNA during the process of transcription.

d.	Any errors made during DNA replication cannot be corrected. If errors are detected after S phase, the cell is destroyed.

19. Mendel's principle of segregation says that:

a.	when gametes are formed, each gamete receives only one allele for a particular gene.

b.	some genes are dominant to others.

c.	a testcross must be used to determine the genotype of an organism with a dominant phenotype.

d.	all of the above

20. Tay-Sachs disease is lethal before reproductive age, but the allele persists in Louisiana French Canadians because:

a.	it is a dominant allele.

b.	new mutations causing this disease are common in that population.

c.	it is not expressed in the phenotype of heterozygous individuals.

d.	the disease is contagious and can be transmitted by nonheritable means.

21. Physical features that are similar in organisms which are not closely related to each other are most likely a result of ____________. A good example of this is the similarities seen between some marsupial and placental mammals.

a.	natural selection.

b.	adaptation to similar environments

c.	convergent evolution

d.	All of the above phrases can be used to correctly fill this blank.

22. In humans, which of the following sex chromosome compliments could be found in males?

23. The proteins that are associated with eukaryotic chromosomes:

a.	help to package the DNA within the nucleus.

b.	are involved in gene expression, through binding with the DNA.

c.	may be replicating or transcribing the DNA.

d.	All of these are true of proteins that are associated with eukaryotic chromosomes.

24. A sequence of DNA nucleotides that contains the information to produce a single protein is a(n):

codon.

gene.

c.	polypeptide.

d.	anticodon.

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Biology 1201A Lecture Notes - Lecture 4: Nitrogenous Base, Chromosome, Sister Chromatids

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