Biology 2581B Lecture Notes - Lecture 9: Maurice Wilkins, Agarose Gel Electrophoresis, Restriction Site
13 Jun 2017
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Document Summary
Major miles stones 1953 discovery of the dna structure. Allow us to modify dna very rapidly, can cut dna in different ways: Restriction enzymes: specific recognition sequences, can differ in length: 4,6, 8 or longer, result in specific cutting patterns, matching sequences. Fragments fit only back together if they have: the same type of ends, e. g. This is true even when it is 2 different strands from two different restriction enzymes: overhang sequences are identical but the whole sequence is not the same. Loss of restriction site when ligating these: fragments must be cut with the same restriction site to retain restriction site. Fragments can be put back together as long as the ends match: putting blunt ends back together, any blunt end will be put together with a blunt end, ligation. The shorter the recognition sequence the more frequently it will cut: different number of fragments, different length of recognition sequences cut.
