Biology 2581B Lecture Notes - Lecture 9: Maurice Wilkins, Agarose Gel Electrophoresis, Recognition Sequence
11 Feb 2017
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Started with knowing what the dna structure is before we can analyze it. James watson (1928 present) and francis crick (1916 2004: they used rosalind franklin"s data. Maurice hugh frederick wilkins (1916 2004) Rosalind franklin (1920-1958: provided the data that made watson and crick come up with the dna structure, died before nobel prize was given for this research. There is a template sequence and there is a recognition sequence that is recognized by a specific restriction enzyme. Blunt ends: enzyme recognizes recognition sequence, snips the dna in the middle and generates a blunt end (straight cut) Sticky ends: different enzymes have different recognition sequences, makes off-centre cut and releases 2 fragments with 2 ends, 5" over hang and 3" over hang, extension is either to 5" or 3" end. Use enzymes for cloning: can cut dna and generate fragments. Restriction enzymes have: specific recognition sequences, recognition sequences differ in length.
