Biology 2382B Lecture Notes - Lecture 3: Electric Field, Luminescence, Cell Membrane
26 Jan 2017
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Isolation and analysis of cell organelles and molecules. Fluorescent activated cell sorting (facs: centrifugation for isolation of intracellular organelles. Electrophoresis and western blotting for analysis of proteins. Recommended readings (from 8th ed. : chapter 1: pg 13, chapter 3: p. 105-108; 112-113, chapter 4: p. 161-163, chapter 7: p. 291. Fluorescent activated cell sorting (facs: cells in suspension/media pass single file through a laser light beam. Cells exit as a droplet tube progressively narrows: both fluorescent light emitted and scattered are measured by detectors. = more cells, proteins, dna, etc. : more fluorescent = more negative, quantification of cells expressing two different cell surface markers by facs. As the cells pass through the facs machine, the intensity of the green and red fluorescence emitted by each cell is recorded. The proportion of each cell population can be calculated. To be considered as expressing two markers, Dots with huge charges of both red and green: cell cycle analysis.
