Biology 2382B Lecture Notes - Lecture 3: Gang Of Youths, Deoxycholic Acid, Chemiluminescence
23 Aug 2016
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Section 3 objectives isolation and analysis of cell organelles and molecules. Labeling live cells with fluorescent antibodies or stains. Antibodies made against specific cell surface proteins can be linked to fluorophores. Membrane permeable fluorescent dyes can be used to label intracellular structures: i. e. hoechst stain binds dna in nucleus cells with bound antibodies or that have taken up the dyes can now be sorted and counted. *note: fluorophore-linked secondary antibodies could also be used in combination with unlabeled primary antibodies. Fluorescent activated cell sorting (facs: cells pass single file through a laser light beam, both fluorescent light emitted and scattered are. 3. measured by detectors individual cells are forced through a nozzle and given a charge proportional to the degree of fluorescence detected: cells with different electric charges are separated by an electric field and collected. *can set the machine to detect specific fluorescent colours (i. e. red and green) Hoechst stain fluorescence intensity of non-dividing cells (g1)
