BCH210H1 Lecture Notes - Lecture 8: Fusion Protein, Catalytic Triad, Globular Protein
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BCH210H1 Full Course Notes
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Ch 2 (q 1(abc), 2(abc), 3, 4, 19b) Western (immuno) blot: genetic engineering of substilisin, mutagenesis of substilisin. Lecture 8/12 - intro to proteins and protein structure. Lecture notes: mutagenesis of substilisin (protein engineering cont. ) Sodium dodecyl sulfate (sds) gel electrophoresis: sds-page: sds-polyacrylamide gel electrophoresis --> powerful technique to sep polypeptides by, sds: denaturing detergent and dissolved membranes and solubilizes proteins their molecular weight (10-15 micrograms) Like urea from earlier --> proteins are inactive as a result. Important detergent used for studying membrane proteins. Structure shown below fyi --> need to know properties. Found in toothpaste, shampoo, etc: lauryl = dodecyl, polyacrylamide: gel matrix to separate proteins, apparatus is shown below --> the smaller the proteins, the easier it will go through the pores. It will go further through the gel as a result. You prepare an extract of all the proteins and put it through this process. Each band can represent one or more proteins of the same weight.
