BCH210H1 Lecture Notes - Lecture 6: Protein Folding, Peptide, Proteasome
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BCH210H1 Full Course Notes
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Ch 2 (q 1(abc), 2(abc), 3, 4, 19b) Protein folding: anifensen"s experiments: rnase, native state of proteins, chaperones. Lecture 6/12 - intro to proteins and protein structure. If protein has 100 aa and each aa had ten conformations, and each conformation was sampled once in 10-13 seconds, it would take 1077 years to fold the protein. But folding takes 103 to 10-1 seconds --> thus folding must be directed: conformation sampling is restricted - recall ramachandran plots, the steps of protein folding is shown below (one model): Secondary structures form first, allowing tertiary structure to form next. Folding of ribonuclease a: since folding is directed, aa must be doing the direction, anfinsen"s postulate: primary sequence determines secondary and tertiary structures. Covalent to h bonds to van der waals: consider all interactions including influence of disulfide bonds, has cysteine residues allowing for disulfide bonds.
